The Extraction and Purification of DNA.
Deoxyribonucleic Acid, commonly referred to as DNA, is the molecule found in everything termed as living. The DNA molecule contains a genetic code that is for every organism, therefore, acting as a blueprint for continuous plans for any substance that the organisms do produce in the course of their lives. Unfortunately as much as it may commonly sound, very few individuals have actually had a chance to see the molecule, this is because the molecule is far too small to easily observe. However, one can simply do extraction on the molecules easily at home or in the school using available household materials like plants and especially peas.
Some of the crucial materials that you may require for the process include alcohol, meat tenderizer, dishwashing detergent mostly in liquid form, a blender, a beaker or rather clear glass container, a stirring rod or a spoon. In the first procedure, blend at least a half cup of split peas in a cupful of water. With that, you should obtain a soupy mixture then use a homogenous solution between the mixtures. One may also consider keeping the extra solution just in case. Secondly, pour some of the solution probably an adequate amount into a container up to a level of at least two inches.
The third step involves adding at least a single spoonful of the liquid dishwashing detergent on top of the solution. Then consider stirring the mixture slowly for about five minutes. Next consider adding a pinch of the meat tenderizer the stir again for five minutes. After stirring the mixture, drizzle some alcohol above the container. One should consider drizzling the alcohol until they obtain a layer of three inches on the split peas solution. Peas are mostly preferred during DNA extractions as they contain this molecule. The use of the detergent is to break down the membrane of the cell hence making it release the contents and the meat tenderizer to dissolve the membrane of the nuclear in order to make it release the DNA inside. The alcohol is used in the process to bring out the DNA strands.
During the purification process, there are a variety of processing kits that can be used. Different samples work with different kind of tissues, plant parts, forensic samples as well as cellular structures. On a daily basis, there are new inventions of machines and cutting edge products through various forms of amplification and facilitations for use in new projects and experiments. Among these ways are the use of magnetic beads and the way happens to be very effective. Another effective way of purification involves the use of plasmid purification kits that enable the production of low endotoxin levels for use in the subject transfection.